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KMID : 0604520090350030193
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Journal of the Society of Cosmetic Scientists of Korea
2009 Volume.35 No. 3 p.193 ~ p.202
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In Vitro Alternative Phototoxicity Test for Various Cosmetic Natural Extracts and Chemicals
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Cho Wan-Goo
Park Jee-Eun
Park Moon-Eok
Lee Sang-Min
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Abstract
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This study was done to assess an alternative method as a replacement of in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals (promethazine, chlorpromazine chlortetracycline, 8-methoxypsoralen, neutral red, bithionol) and non-phototoxic materials (cinnamic aldehyde, p-aminobenzoic acid, sodium lauryl sulfate, L-cysteine). The cell viability was measured by neutral red uptake (NRU) assay. The results of the NRU phototoxicity (PT) assay showed a close agreement with in vivo test except bithionol. We also have tested the cosmetic ingredients including Mediminⓡ A, Mediminⓡ D, LGⓡ 106W, Phytoclearⓡ EL-1, Carex humilis L. extract, Canna indica L. extract, Salvia miltiorrhira Bunge extract, Parsolⓡ MCX and Parsolⓡ 1789. Most materials except Salvia miltiorrhira Bunge extract did not show any phototoxicity.
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KEYWORD
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phototoxicity, neutral red uptake, cell viability, fibroblast, cosmetic
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